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gapdh snrpn gfp reporter cell line  (Addgene inc)


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    Addgene inc gapdh snrpn gfp reporter cell line
    Epigenetic silencing <t>of</t> <t>the</t> <t>Gapdh-Snrpn-GFP</t> reporter and endogenous CD151 in HEK293T cells using plasmid-based CRISPR OFF-EEs (A) Schematic of the epigenetic silencing strategy (SpCas9/SF01-OFF-EE). The Gapdh-Snrpn-GFP reporter is targeted by a catalytically inactive Cas protein (dSpCas9 or dSF01) fused to epigenetic editor (EE) domains (DNMT3A-3L and KRAB). The complex is directed by a specific single guide RNA (sgRNA or crRNA) to the Snrpn promoter, leading to targeted DNA methylation and silencing of GFP expression. (B) Representative flow cytometry plots showing GFP expression 30 days post-transfection with the indicated editor plasmids and a single corresponding gRNA. (C) Quantification of GFP silencing from experiments in (B). Data represent the mean ± SD of three independent biological replicates. (D) Methylation levels of individual CpG sites within the targeted Snrpn promoter and the non-targeted adjacent Gapdh promoter, confirming the specificity of methylation. Data are the mean ± SD ( n = 3). (E) Quantification of CD151 surface protein knockdown in HEK293T cells 30 days after transfection with editor plasmids and a pool of three guides. Data represent the mean ± SD ( n = 3). (F) Methylation levels of individual CpG sites within the targeted CD151 promoter. Data are the mean ± SD ( n = 3).
    Gapdh Snrpn Gfp Reporter Cell Line, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gapdh+snrpn+gfp+reporter+cell+line/GAPDH-CPG-TV+(Plasmid+%2370148)/pmc12957561-37-6-14
    Average 93 stars, based on 3 article reviews
    gapdh snrpn gfp reporter cell line - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo"

    Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo

    Journal: The Innovation

    doi: 10.1016/j.xinn.2025.101151

    Epigenetic silencing of the Gapdh-Snrpn-GFP reporter and endogenous CD151 in HEK293T cells using plasmid-based CRISPR OFF-EEs (A) Schematic of the epigenetic silencing strategy (SpCas9/SF01-OFF-EE). The Gapdh-Snrpn-GFP reporter is targeted by a catalytically inactive Cas protein (dSpCas9 or dSF01) fused to epigenetic editor (EE) domains (DNMT3A-3L and KRAB). The complex is directed by a specific single guide RNA (sgRNA or crRNA) to the Snrpn promoter, leading to targeted DNA methylation and silencing of GFP expression. (B) Representative flow cytometry plots showing GFP expression 30 days post-transfection with the indicated editor plasmids and a single corresponding gRNA. (C) Quantification of GFP silencing from experiments in (B). Data represent the mean ± SD of three independent biological replicates. (D) Methylation levels of individual CpG sites within the targeted Snrpn promoter and the non-targeted adjacent Gapdh promoter, confirming the specificity of methylation. Data are the mean ± SD ( n = 3). (E) Quantification of CD151 surface protein knockdown in HEK293T cells 30 days after transfection with editor plasmids and a pool of three guides. Data represent the mean ± SD ( n = 3). (F) Methylation levels of individual CpG sites within the targeted CD151 promoter. Data are the mean ± SD ( n = 3).
    Figure Legend Snippet: Epigenetic silencing of the Gapdh-Snrpn-GFP reporter and endogenous CD151 in HEK293T cells using plasmid-based CRISPR OFF-EEs (A) Schematic of the epigenetic silencing strategy (SpCas9/SF01-OFF-EE). The Gapdh-Snrpn-GFP reporter is targeted by a catalytically inactive Cas protein (dSpCas9 or dSF01) fused to epigenetic editor (EE) domains (DNMT3A-3L and KRAB). The complex is directed by a specific single guide RNA (sgRNA or crRNA) to the Snrpn promoter, leading to targeted DNA methylation and silencing of GFP expression. (B) Representative flow cytometry plots showing GFP expression 30 days post-transfection with the indicated editor plasmids and a single corresponding gRNA. (C) Quantification of GFP silencing from experiments in (B). Data represent the mean ± SD of three independent biological replicates. (D) Methylation levels of individual CpG sites within the targeted Snrpn promoter and the non-targeted adjacent Gapdh promoter, confirming the specificity of methylation. Data are the mean ± SD ( n = 3). (E) Quantification of CD151 surface protein knockdown in HEK293T cells 30 days after transfection with editor plasmids and a pool of three guides. Data represent the mean ± SD ( n = 3). (F) Methylation levels of individual CpG sites within the targeted CD151 promoter. Data are the mean ± SD ( n = 3).

    Techniques Used: Plasmid Preparation, CRISPR, DNA Methylation Assay, Expressing, Flow Cytometry, Transfection, Methylation, Knockdown

    Related Articles

    Plasmid Preparation:

    Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo
    Article Snippet: .. The lentiviral plasmid for generating the Gapdh-Snrpn-GFP reporter cell line was also sourced from Addgene (Addgene, #70148). .. All plasmids intended for transient transfection experiments were purified using a kit that includes an endotoxin removal step (ZymoPURE Plasmid Miniprep Kit, Zymo Research).

    Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
    Article Snippet: .. The 99 lentiviral plasmid for generating the Gapdh-Snrpn-GFP reporter cell line was also sourced from 100 Addgene (Addgene, #70148). .. All plasmids intended for transient transfection experiments were 101 purified using a kit that includes an endotoxin removal step (ZymoPURE Plasmid Miniprep Kit, 102 Zymo Research).



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    gapdh snrpn gfp reporter cell line  (Addgene inc)
    93
    Addgene inc gapdh snrpn gfp reporter cell line
    Epigenetic silencing <t>of</t> <t>the</t> <t>Gapdh-Snrpn-GFP</t> reporter and endogenous CD151 in HEK293T cells using plasmid-based CRISPR OFF-EEs (A) Schematic of the epigenetic silencing strategy (SpCas9/SF01-OFF-EE). The Gapdh-Snrpn-GFP reporter is targeted by a catalytically inactive Cas protein (dSpCas9 or dSF01) fused to epigenetic editor (EE) domains (DNMT3A-3L and KRAB). The complex is directed by a specific single guide RNA (sgRNA or crRNA) to the Snrpn promoter, leading to targeted DNA methylation and silencing of GFP expression. (B) Representative flow cytometry plots showing GFP expression 30 days post-transfection with the indicated editor plasmids and a single corresponding gRNA. (C) Quantification of GFP silencing from experiments in (B). Data represent the mean ± SD of three independent biological replicates. (D) Methylation levels of individual CpG sites within the targeted Snrpn promoter and the non-targeted adjacent Gapdh promoter, confirming the specificity of methylation. Data are the mean ± SD ( n = 3). (E) Quantification of CD151 surface protein knockdown in HEK293T cells 30 days after transfection with editor plasmids and a pool of three guides. Data represent the mean ± SD ( n = 3). (F) Methylation levels of individual CpG sites within the targeted CD151 promoter. Data are the mean ± SD ( n = 3).
    Gapdh Snrpn Gfp Reporter Cell Line, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gapdh+snrpn+gfp+reporter+cell+line/GAPDH-CPG-TV+(Plasmid+%2370148)/pmc12957561-37-6-14
    Average 93 stars, based on 1 article reviews
    gapdh snrpn gfp reporter cell line - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Epigenetic silencing of the Gapdh-Snrpn-GFP reporter and endogenous CD151 in HEK293T cells using plasmid-based CRISPR OFF-EEs (A) Schematic of the epigenetic silencing strategy (SpCas9/SF01-OFF-EE). The Gapdh-Snrpn-GFP reporter is targeted by a catalytically inactive Cas protein (dSpCas9 or dSF01) fused to epigenetic editor (EE) domains (DNMT3A-3L and KRAB). The complex is directed by a specific single guide RNA (sgRNA or crRNA) to the Snrpn promoter, leading to targeted DNA methylation and silencing of GFP expression. (B) Representative flow cytometry plots showing GFP expression 30 days post-transfection with the indicated editor plasmids and a single corresponding gRNA. (C) Quantification of GFP silencing from experiments in (B). Data represent the mean ± SD of three independent biological replicates. (D) Methylation levels of individual CpG sites within the targeted Snrpn promoter and the non-targeted adjacent Gapdh promoter, confirming the specificity of methylation. Data are the mean ± SD ( n = 3). (E) Quantification of CD151 surface protein knockdown in HEK293T cells 30 days after transfection with editor plasmids and a pool of three guides. Data represent the mean ± SD ( n = 3). (F) Methylation levels of individual CpG sites within the targeted CD151 promoter. Data are the mean ± SD ( n = 3).

    Journal: The Innovation

    Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo

    doi: 10.1016/j.xinn.2025.101151

    Figure Lengend Snippet: Epigenetic silencing of the Gapdh-Snrpn-GFP reporter and endogenous CD151 in HEK293T cells using plasmid-based CRISPR OFF-EEs (A) Schematic of the epigenetic silencing strategy (SpCas9/SF01-OFF-EE). The Gapdh-Snrpn-GFP reporter is targeted by a catalytically inactive Cas protein (dSpCas9 or dSF01) fused to epigenetic editor (EE) domains (DNMT3A-3L and KRAB). The complex is directed by a specific single guide RNA (sgRNA or crRNA) to the Snrpn promoter, leading to targeted DNA methylation and silencing of GFP expression. (B) Representative flow cytometry plots showing GFP expression 30 days post-transfection with the indicated editor plasmids and a single corresponding gRNA. (C) Quantification of GFP silencing from experiments in (B). Data represent the mean ± SD of three independent biological replicates. (D) Methylation levels of individual CpG sites within the targeted Snrpn promoter and the non-targeted adjacent Gapdh promoter, confirming the specificity of methylation. Data are the mean ± SD ( n = 3). (E) Quantification of CD151 surface protein knockdown in HEK293T cells 30 days after transfection with editor plasmids and a pool of three guides. Data represent the mean ± SD ( n = 3). (F) Methylation levels of individual CpG sites within the targeted CD151 promoter. Data are the mean ± SD ( n = 3).

    Article Snippet: The lentiviral plasmid for generating the Gapdh-Snrpn-GFP reporter cell line was also sourced from Addgene (Addgene, #70148).

    Techniques: Plasmid Preparation, CRISPR, DNA Methylation Assay, Expressing, Flow Cytometry, Transfection, Methylation, Knockdown